This is kind of huge, given how Title IX has generally been interpreted pretty narrowly against trans and gender non-conforming people:

Federal prosecutors have used a novel interpretation of the Title IX statue, which prohibits discrimination against students on the basis of gender, to help broker a settlement in a lawsuit brought by a gay teen against his upstate New York school district, NPR’s Ari Shapiro tells us.

The lawsuit involved a boy who was bullied for being effeminate.

This is the first time since the Clinton Administration that the Justice Department has claimed that Title IX applies to discrimination based gender presentation, beyond simple discrimination based on sex.